VERTICAL CHROMATOGRAPHY : Filtrations Products Syringe Filter : Choosing Filters

Home

products > Filtration Products > Choosing Filters

About Us

News

There are many different types of filters available from Vertical. Generally there are four main points to consider when choosing the filters. These are :
Burst Pressure (the more burst pressure the more housing integrity and prevent leakage)
Sample volume
Membrane type
Solvent/Membrane compatibility.

1. Burst pressure is the maximum pressure to force air through a closed outlet end casing.
2. Retain volume is a volume of solvent retained inside the casing.

Syringe Filter Housing Specification
Size	Burst Pressure¹	Retain Volume²	Sample Volume
4mm	75 psi	8 µl	≤ 2 ml
13mm	100 psi	30 µl	≤ 6 ml
17mm	100 psi	45 µl	≤ 40 ml
25mm	100 psi	120 µl	≤ 70 ml

Membrane Selection Guide

Membrane Type	Applications
Nylon Nylon (Polyamide) is hydrophilic, good olvent resistance and medium protein binding.	• Filtration of all aqueous and most solvent based samples • Use with care for biological compounds
PTFE PTFE (Polytetrafluoroethylene) is hydrophobic, highest solvent resistance and high protein binding.	• Filtration of non-aqueous or solvent based samples • Good for filtering and degassing chromatography
PVDF, Hydrophilic Hydrophilic PVDF (Polyvinylidene difluoride) is high solvent resistance, low protein binding and good flowrate characteristics.	• Filtration of all aqueous and most solvent based samples • General filtration of biological samples
PVDF, hydrophobic Hydrophobic PVDF (Polyvinylidene Fluoride) is high solvent resistance, low protein binding and good flowrate characteristics.	• Filtration of non-aqueous and most solvent based samples • General filtration of biological samples
CA CA (Cellulose Acetate) is hydrophilic, limited solvent resistance and very low protein binding.	• Ideal for aqueous based samples • Ideal for tissue culture and sensitive biological samples.
RC RC (Regenerated Cellulose) is hydrophilic, high solvent resistance and lowest protein binding.	• Ideal for aqueous based samples • General filtration of biological samples
MCE MCE (Mixed Cellulose Ester) is hydrophilic, limit solvent resistance, high protein binding and excellent flowrate characteristics.	• Ideal for aqueous based samples • Specifically filtration of trace protein contamination
Polypropylene PP (Polypropylene) is hydrophobic, high solvent resistance and lower protein binding than PVDF.	• Filtration of all aqueous and most solvent based samples • General filtration of biological samples
PES PES (Polyethersulfone) is hydrophilic, limited solvent resistance, low protein binding and good flow rate characteristics.	• Ideal for aqueous based samples • Ideal for tissue culture and sensitive biological samples.
GMF (Glass Microfiber) GMF (Glass microfiber) is commonly used as pre-filters to remove large particulates and minimized sample loss.	• General filtration of biological samples • Used in a variety of sample clean-up and perfiltration.