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  Products > GC Capillary Columns > VertiBound™ Quality

Column Reproducibility and Specifications
     By characterizing each VertiBond™ column type with specifications on key chromatographic parameters, Vertical guarantees the columns that you receive are reproducible. This ensures that methods can be reproduced on columns from different batche and different laboratories.

Maximum Temperature
     The maximum temperature values that a column can be operated are given:
     - The lower level is the maximum isothermal temperature limit and is related to the bleed level specifications.
     - The higher level is the maximum programmed temperature limit and the maximum temperature at which the column can be cleaned or conditioned.

Efficiency (N/m)
     The efficiency of a column is a measure of the separation power. Narrow-bore columns, which have a higher plate number per meter, have a higher separation power. This results in a better separation of two peaks in shorter analysis times.

Retention Index (RI)
     Retention index is the measure of a column’s selectivity and therefore determines the elution order. A column with a small variation will have a high column-to-column reproducibility.

Inertness (As)
     The inertness of a column influences the elution and recovery of polar (active) compounds. The high inertness column provides the better peak shape and therefore the more accurately the peak can be quantified. This results in more reliable analytical outputs.

Bleed (pA)
     The bleed level of a column is a measure of its stability and sensitivity. Low bleed columns are therefore more suitable for trace analysis, especially in combination with mass spectrometers, and have a longer column lifetime.

Low Bleed Columns
    The bleed level of a column is a measure of its stability and sensitivity. Bleed is directly propotional to the amount of stationary phase present and exponentially proportional to temperature, hence, the elevated baseline as the temperature rises to the column’s upper temperature limit.
     The low bleed columns have superior analytical sensivity. Therefore, they are more suitable for trace analysis where
sensivity and instrument performance are issues should be concerned about bleed. Many GC detectors (MS, FID, ECD, NPD, SCD) are sensitive to contamination from bleed and will require less maintenance when using low bleed columns.

“ms” Columns
    In analytical Laboratories, GC/MS instruments are use to analyze a broadening range of trace level, higher temperature samples. These samples require increasingly inert, lower bleed, higher temperature columns. It seems logical and proper to apply the “ms” name to columns deliberately designed to chromatograph a broader range of low level samples and generate minimal lower bleed at higher temperatures. Vertical® allows GC/MS analysts a wide range of stationary phase “ms” columns, while guaranteeing low column bleed. Each VertiBond™ “ms” column is tested and ensured that exceeds requirements of the most sensitive mass spectrometers.




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