VERTICAL CHROMATOGRAPHY CO.,LTD. : HPLC COLUMNS Guard Holder Guard Cartridges Choosing Columns

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Column Length
Shorter columns offer faster run times. Longer columns offer greater resolution. 10-50 mm length is recommended for fast separations, method development. 150mm length is recommended for general separations. 250mm length is recommended for high resolution separations.

Column i.d.
The standard column i.d. is 4.6mm. Narrower columns allow smaller sample sizes, and reduce mobile phase flow rates. Wider columns allow for larger sample sizes and minimize the system’s
dead volume.

hplc columns

HPLC Column Formats
Vertical offers HPLC columns in several different formats for different applications.
A. Analytical Columns – 50 to 300mm lengths, 4.6mm i.d.
B. Short Columns – 10 or 50mm lengths, 4.6mm i.d
C. Narrow-Bore Columns – 50 to 300mm lengths, 3.0mm i.d
    Smaller i.d. significantly reduces solvent usage. Compatible with both APCI and ESI.
D. Capillary and LC/MS Columns – 50 to 300mm lengths, 150μm-2.1mm i.d.s Small-bore columns
    compatible with LC/MS instrumentation.
E. Preparative Columns – 50 to 300mm lengths, 7-50mm i.d.s
    High volume and mass loading capacity.
F. PEEK Columns – 50 to 300mm lengths, 2.1, 4.6mm i.d.
    Complete Bio-compatibilty and IC applications
G. Guard Cartridges – 7.5mm length 2.1, 3.2, 4.6mm i.d.
    The Guard System has disposable cartridges for quick and easy replacement.

VertiSep^™ Vertical manufactures our columns to the highest standards of quality and reproducibility with our technical expertise for the best columns, the best applications, and the best technical support.
VertiSep^™ UPS Ultra High Purity Silica – Vertical 's Top series VertiSep UPS phases are made from Ultra-high purity silica. Eliminate bleed and tailing, and choose from a full selection of phases and formats to optimize your retention and resolution.
VertiSep^™ AQS Reversed-phase columns for use with 100% Aqueous mobile phases VertiSep AQS phases are compatible with a full range of aqueous mobile phases for excellent retention of highly polar analytes.
VertiSep^™ HCS Highest Carbon loading Reverse-Phase Columns for high resolution applications VertiSep HCS phases are highest carbon loading, polymeric bonding and endcapping for highest resolution and long column lifetimes
VertiSep^™ BDS Base-Deactivated Silica- the equivalent for Hypersil BDS VertiSep BDS phase has physical and performance characteristics very similar to those of Hypersil BDS and exellent support for analysis of basic compounds.
VertiSep^™ BIO Special packings for protein and life science applications VertiSep BIO large-pore phases are ideal for large molecules, a variety of life science and proteomic applications.
VertiSep^™ GES General Purpose, economical columns VertiSep GES phases provide performance and reliability at an exceptional price. easily scale-up from analytical to affordable prep columns. Broad pH stability and high efficiency make it an economical general purpose applications
VertiSep^™ SUGAR Special packings for sugar or carbohydrate applications VertiSep Carbohydrate are polymer-based packing for separation of sugars or carbohydrate.
VertiSep^™ OA Special packings for organic acids by ion exclusion chromatography VertiSep OA are polymer-based packing for separation of weakly ionizable species such as organic acids and alcohols by ion exclusion chromatography.
VertiSep^™ NH2 Special packings for amino acids by Cation exhange chromatography VertiSep AMINO are polymer-based packing for high efficiency and high resolution separation of amino acids by cation exchange chromatography. Available for both complex physiological fluids and protein hydrolysate amino acids.
VertiSep^™ IC Special packings for ions by ion exchange chromatography VertiSep IC are polymer-based packing for inorganic or organic anions and cations by ion exchange chromatography followed by suppressed or non-suppressed conductivity detection.
VertiSep^™ PRP Special packings for use with samples or mobile phase pH range 0-14. VertiSep PRP are polymer-based packing for samples or mobile phase pH meter 0-14 by reverse-phase chromatography it is very rugged and long. Lasting matreials.